Department of Transfusion Medicine
Laboratory Services
    Blood Processing and Issue Laboratory
    Blood Component Laboratory
    Transfusion Transmitted Infection Screening Laboratory
    Immunohematology Laboratory
    Quality Control Laboratory
     

    Blood Processing Laboratory
    This laboratory accepts requests for blood and blood products for patients from the Institute's hospital as well as those from King George's Medical College, Command Hospital, Balrampur Hospital and Civil Hospital in Lucknow. The facility is licensed by the Drugs Controller of India. 

    Activities of this laboratory include: 

    Donor blood processing: Tests for determining the ABO group & Rh type, detecting unexpected antibodies to red cell antigens, confirmation of Rh negative status by genotyping & Du testing, resolving ABO discrepancies, etc. 

    Cross matching: Routine tests include ABO grouping, Rh typing, antibody screening and identification. Requests for routine cross match for planned transfusion are processed with full four-test-tube cross-match including enzyme, albumin and auto-control. Tests for cold antibodies are done when necessary, viz for patients scheduled for open heart surgery, renal transplantation, etc. 

    Issue of blood and components:  Blood and blood components are verified and issued to nursing wards and operating rooms; product modifications (irradiation etc) are performed and final disposition of products are documented.
     

    Blood Component Laboratory
    Blood is a precious human resource. To exercise maximum economy in its use, every unit of blood is separated into various components, such as packed red cells, fresh frozen plasma, platelets, cryoprecipitate and cryo-deficient plasma, etc. Buffy coat reduced red cell and platelets are also produced.  The component separation program is fully supported by National AIDS Control Organization. The laboratory has state-of-the-art equipment and technology. Spare plasma generated by this activity is sent to National Plasma Fractionation Center (NPFC), Mumbai for further use. The laboratory also supports the Peripheral Blood Stem Cell program of the Institute by providing gamma-irradiated blood and blood products, and help in cryo-preservation of stem cells. 

    Transfusion Transmitted Infection Screening Laboratory
    Provision of safe blood is of utmost priority. Every unit of blood & component is screened for HbsAg, anti-HIV 1&2, anti-HCV and VDRL. The laboratory is fully automated and all four infectious markers can be screened on the day of collection. 
     

    Immunohematology Laboratory
    The department has a well-equipped laboratory to provide diagnostic aids in the management of various autoimmune disorders such as auto immune hemolytic anemia, paroxysmal nocturnal hematuria, cryoglobulinemia, red cell serology of SLE etc. Besides this, the laboratory is responsible for investigations of transfusion reactions & management of Rh-negative antenatal case. Various investigations that are performed include, DAT, IAT, elution, antibody screening, antibody identification & titer, serological characterization of AIHA, Saliva screening test, Cold antibodies etc. 
     

    Quality Control Laboratory
    To achieve objectives of supply of quality and safe blood, strict adherence to quality control at all phases of donor selection, screening & processing of blood & serological procedures for grouping and typing of blood are being followed routinely. All reagents, antisera, blood components and equipment are subjected to strict quality checks at periodic intervals according to the standards. 

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